噬菌体φX174 DNA的核苷酸序列。
Nucleotide sequence of bacteriophage phi X174 DNA.
作者信息
Sanger F, Air G M, Barrell B G, Brown N L, Coulson A R, Fiddes C A, Hutchison C A, Slocombe P M, Smith M
出版信息
Nature. 1977 Feb 24;265(5596):687-95. doi: 10.1038/265687a0.
DOI:10.1038/265687a0
PMID:870828
Abstract
A DNA sequence for the genome of bacteriophage phi X174 of approximately 5,375 nucleotides has been determined using the rapid and simple 'plus and minus' method. The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Two pairs of genes are coded by the same region of DNA using different reading frames.
摘要
利用快速简便的“加减法”方法，已确定了噬菌体φX174基因组约5375个核苷酸的DNA序列。该序列确定了许多负责该生物体九个已知基因蛋白质产生的特征，包括蛋白质和RNA的起始和终止位点。两对基因由DNA的同一区域使用不同的阅读框编码。
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