Moro F, Goñi F M, Urbaneja M A
Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
FEBS Lett. 1993 Sep 13;330(2):129-32. doi: 10.1016/0014-5793(93)80257-u.
Studies of fluorescence quenching in membrane proteins are complicated by the fact that the barrier effect of the bilayer towards the quenchers is not known with precision. Our studies show that (a) both acrylamide and iodide can permeate the membrane at comparable rates, (b) when quenchers are added externally to a vesicle suspension, the apparent Stern-Volmer quenching constants for the same fluorophores are lower in the inner than in the outer aqueous compartments, and (c) at least some non-polar fluorophores embedded in the bilayer are quenched by iodide, but not by acrylamide.
膜蛋白中荧光猝灭的研究因双层膜对猝灭剂的屏障效应无法精确得知而变得复杂。我们的研究表明：（a）丙烯酰胺和碘离子都能以相当的速率透过膜；（b）当向囊泡悬浮液外部添加猝灭剂时，相同荧光团在内部水相区室中的表观斯特恩-沃尔默猝灭常数低于外部水相区室；（c）嵌入双层膜中的至少一些非极性荧光团会被碘离子猝灭，但不会被丙烯酰胺猝灭。
