Gaggero C, Moreno F, Laviña M
División Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
J Bacteriol. 1993 Sep;175(17):5420-7. doi: 10.1128/jb.175.17.5420-5427.1993.
The microcin H47 genetic determinants span a DNA region of ca. 10 kb and represent the first description of an enterobacterial antibiotic system located in the chromosome of the producing strain. Transcriptional and translational fusions to lacZ showed a complex transcriptional organization of the microcin H47 system. Complementation tests identified six genes that are necessary for the production of the antibiotic; the products of two of them are involved in the export of microcin to the extracellular medium. The immunity determinant was located in an 0.8-kb DNA fragment. There is a putative "silent region" of ca. 3 kb inside the system that could not be clearly related to any antibiotic function. Protein products were identified and assigned to three production genes and also to a gene from the silent region.
微菌素H47的遗传决定簇跨越约10 kb的DNA区域，是对位于产生菌染色体上的一种肠杆菌抗生素系统的首次描述。与lacZ的转录融合和翻译融合显示了微菌素H47系统复杂的转录组织。互补试验确定了六个对抗生素产生所必需的基因；其中两个基因的产物参与微菌素向细胞外培养基的输出。免疫决定簇位于一个0.8 kb的DNA片段中。该系统内有一个约3 kb的假定“沉默区域”，其与任何抗生素功能均无明显关联。已鉴定出蛋白质产物，并将其对应到三个产生基因以及沉默区域的一个基因。
