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HeLa肿瘤细胞中核仁蛋白的ADP核糖基化作用

ADP-ribosylation of nucleolar proteins in HeLa tumor cells.

作者信息

Leitinger N, Wesierska-Gadek J

机构信息

Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.

出版信息

J Cell Biochem. 1993 Jun;52(2):153-8. doi: 10.1002/jcb.240520207.

DOI:10.1002/jcb.240520207
PMID:8366133
Abstract

ADP-ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32P-NAD; then the nucleolar proteins were analyzed by 1-dimensional and 2-dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE-cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP-ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP-ribosylation.

摘要

对指数生长期的HeLa细胞核仁中的ADP-核糖基化反应进行了研究。用32P-NAD标记分离的细胞核或核仁；然后通过一维和二维聚丙烯酰胺凝胶电泳（PAGE）分析核仁蛋白，并通过放射自显影检测修饰的蛋白。标记的核仁蛋白也在DEAE-纤维素上进行色谱分离。对总核仁蛋白和色谱纯化蛋白的电泳分析表明，除了核ADP-核糖基转移酶和组蛋白外，两种特征性的核仁磷蛋白核基质蛋白/B23和核仁素/C23也被ADP-核糖基化修饰。

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ADP-ribosylation of nucleolar proteins in HeLa tumor cells.HeLa肿瘤细胞中核仁蛋白的ADP核糖基化作用
J Cell Biochem. 1993 Jun;52(2):153-8. doi: 10.1002/jcb.240520207.
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ADP-ribosylation of heterogeneous ribonucleoproteins in HeLa cells.HeLa细胞中异质性核糖核蛋白的ADP核糖基化作用
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[ADP-ribosylation of nuclear proteins in the rat brain].
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Modification of nucleolar protein B23 after exposure to ionizing radiation.暴露于电离辐射后核仁蛋白B23的修饰。
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