Pawlowska M, Chen S, Armstrong D W
Department of Chemistry, University of Missouri-Rolla 65401.
J Chromatogr. 1993 Jul 9;641(2):257-65. doi: 10.1016/0021-9673(93)80142-u.
A new derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), has been used successfully for chromatographic enantioseparation of 31 amino acids on cyclodextrin bonded stationary phases. AQC reacts with both primary and secondary amino acids to produce stable and highly fluorescent derivatives suitable for efficient and sensitive chromatographic determinations. The derivatization reaction proceeds without detectable racemization. The detection limit is in the femtomole range and approximately 0.0075% of the D-enantiomer in an excess of the L-enantiomer is detectable. High resolution values are needed when determining trace enantiomeric impurities.
一种新型衍生试剂，6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯（AQC），已成功用于在环糊精键合固定相上对31种氨基酸进行色谱对映体分离。AQC与伯氨基酸和仲氨基酸都发生反应，生成稳定且高荧光的衍生物，适用于高效、灵敏的色谱测定。衍生化反应进行时无明显的消旋化现象。检测限在飞摩尔范围内，在过量的L-对映体中，约0.0075%的D-对映体可被检测到。在测定痕量对映体杂质时需要高分辨率值。
