Saha M, Saha J, Giese R W
Department of Pharmaceutical Sciences, Bouve Pharmacy and Health Sciences, Northeastern University, Boston, MA 02115.
J Chromatogr. 1993 Jul 9;641(2):400-4. doi: 10.1016/0021-9673(93)80158-5.
4-(Trifluoromethyl)-2,3,5,6-tetrafluorobenzyl bromide (TTBB) was synthesized in a single step from alpha,alpha,alpha,2,3,5,6-heptafluoro-p-xylene. The purpose of TTBB is to function as an analogue of pentafluorobenzyl bromide (PFBB) in electrophoric derivatization reactions prior to detection by gas chromatography-electron-capture negative ion mass spectrometry (GC-ECNI-MS). In more detail, it was anticipated that TTBB could be used along with, or as a substitute for, PFBB to help control some interferences and confirm results. This is because a TTBB-product (of an analyte) would have different retention and sometimes m/z characteristics than a corresponding PFBB product in GC-ECNI-MS, while the two products should be similar in their ease of formation and yields. Results demonstrating these expectations were achieved by derivatizing and detecting two analytes with these reagents: N7-(2-hydroxyethyl)xanthine, and 2,3-pyrenedicarboxylic acid.
4-（三氟甲基）-2,3,5,6-四氟苄基溴（TTBB）由α,α,α,2,3,5,6-七氟对二甲苯一步合成。TTBB的用途是在气相色谱-电子捕获负离子质谱（GC-ECNI-MS）检测之前，作为五氟苄基溴（PFBB）的类似物用于电泳衍生化反应。更详细地说，预计TTBB可与PFBB一起使用或替代PFBB，以帮助控制一些干扰并确认结果。这是因为在GC-ECNI-MS中，（分析物的）TTBB产物与相应的PFBB产物相比，具有不同的保留时间，有时m/z特征也不同，而这两种产物在形成的难易程度和产率方面应该相似。通过用这些试剂衍生化并检测两种分析物：N7-（2-羟乙基）黄嘌呤和2,3-芘二甲酸，得到了证明这些预期的结果。
