Rodriguez L A, Ellis A E, Nieto T P
Department of Fundamental Biology, Faculty of Science, Orense, Spain.
Microb Pathog. 1993 May;14(5):411-5. doi: 10.1006/mpat.1993.1040.
The purified acetylcholinesterase (AcChE) toxin, crude extracellular products (ECP) or viable virulent Aeromonas hydrophila were injected intraperitoneally into rainbow trout in different sublethal and lethal doses. When fish showed signs of morbidity, brain tissue was excised and assayed for acetylcholinesterase activity. In all cases there was a large increase in AcChE activity (about 40-fold for purified AcChE-toxin). This was shown to be due to an accumulation of the fish's own AcChE and not the bacterial toxin. Nevertheless, the latter was detected in brain homogenates from fish in all treatment groups using a rabbit antiserum to the purified toxin to probe Western blots of brain homogenates, demonstrating that the toxin does gain access to brain tissue and is produced during in vivo infection. The results strongly suggest that this toxin plays a central role in the pathogenesis of A. hydrophila infection.
将纯化的乙酰胆碱酯酶（AcChE）毒素、粗细胞外产物（ECP）或活的强毒嗜水气单胞菌以不同的亚致死剂量和致死剂量腹腔注射到虹鳟鱼体内。当鱼出现发病迹象时，切除脑组织并检测乙酰胆碱酯酶活性。在所有情况下，乙酰胆碱酯酶活性都大幅增加（纯化的AcChE毒素组约增加40倍）。结果表明，这是由于鱼自身的乙酰胆碱酯酶积累所致，而非细菌毒素。然而，使用针对纯化毒素的兔抗血清对脑匀浆进行蛋白质印迹检测，在所有处理组的鱼脑匀浆中均检测到了细菌毒素，这表明该毒素确实能够进入脑组织并在体内感染过程中产生。结果强烈表明，这种毒素在嗜水气单胞菌感染的发病机制中起核心作用。
