嗜水气单胞菌AH-3 AexT是一种通过III型分泌系统分泌的ADP核糖基化毒素。
Aeromonas hydrophila AH-3 AexT is an ADP-ribosylating toxin secreted through the type III secretion system.
作者信息
Vilches Silvia, Wilhelms Markus, Yu Hong Bing, Leung Ka Yin, Tomás Juan M, Merino Susana
机构信息
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, Barcelona, Spain.
出版信息
Microb Pathog. 2008 Jan;44(1):1-12. doi: 10.1016/j.micpath.2007.06.004. Epub 2007 Jul 1.
We cloned and sequenced an ADP-ribosylating toxin (AexT) from a mesophilic Aeromonas hydrophila strain AH-3 with a type III secretion system (T3SS). This toxin only showed homology, in genes and proteins, with the first half of A. salmonicida AexT. The A. hydrophila AexT showed ADP-ribosyltransferase activity, translocation through the T3SS system, and this A. hydrophila T3SS system is inducible under calcium-depleted conditions. The A. hydrophila aexT mutant showed a slight reduction in their virulence assayed by several methods when compared to the wild-type strain, while an A. hydrophila T3SS mutant is highly reduced in virulence on the same assays. The A. hydrophila AexT is the first described and the smallest T3SS effector toxin found in mesophilic Aeromonas with a functional T3SS.
我们从一株具有III型分泌系统（T3SS）的嗜温嗜水气单胞菌菌株AH-3中克隆并测序了一种ADP-核糖基化毒素（AexT）。该毒素在基因和蛋白质水平上仅与杀鲑气单胞菌AexT的前半部分具有同源性。嗜水气单胞菌AexT具有ADP-核糖基转移酶活性，可通过T3SS系统进行转运，并且该嗜水气单胞菌T3SS系统在缺钙条件下可被诱导。与野生型菌株相比，通过多种方法测定，嗜水气单胞菌aexT突变体的毒力略有降低，而在相同测定中，嗜水气单胞菌T3SS突变体的毒力则大幅降低。嗜水气单胞菌AexT是在具有功能性T3SS的嗜温气单胞菌中首次描述且最小的T3SS效应毒素。
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