Miller M, Kawabata S, Wiltshire-Clement M, Reventos J, Gordon J W
Department of Geriatrics and Adult Development, Mount Sinai Medical Center, New York, NY 10029.
Neuroendocrinology. 1993 Apr;57(4):621-5. doi: 10.1159/000126416.
The rat arginine vasopressin (AVP) genomic sequence has been utilized to develop a line of transgenic mice homozygous and heterozygous for the transgene. Expression of the rat AVP gene was demonstrated by Southern blotting and resulted in increased amounts of AVP in hypothalamus and frontotemporal brain cortex. Secretion of AVP from the neurohypophysial system results in an increased concentration of the hormone in the plasma and in an increased excretion in the urine in amounts three to five times those of normal mice. Extraneural ectopic hormone production was found only in the pancreas. Despite chronic hypersecretion of AVP, 24-hour urine volume and osmolality did not show evidence of increased antidiuretic hormone action on the kidney, so that, under basal conditions, the water balance in the animals is unaffected.
大鼠精氨酸加压素（AVP）基因组序列已被用于培育转基因纯合子和杂合子小鼠品系。通过Southern印迹法证实了大鼠AVP基因的表达，结果导致下丘脑和额颞脑皮质中AVP含量增加。神经垂体系统分泌的AVP导致血浆中该激素浓度升高，尿液排泄量增加，是正常小鼠的三到五倍。仅在胰腺中发现了神经外异位激素产生。尽管AVP长期分泌过多，但24小时尿量和渗透压并未显示抗利尿激素对肾脏的作用增强，因此在基础条件下，动物的水平衡未受影响。
