过量的聚合酶链式反应（PCR）引物可能会显著影响单链构象多态性（SSCP）的效率。 - Suppr

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超能文献

Excess PCR primers may dramatically affect SSCP efficiency.

作者信息

Cai Q Q, Touitou I

机构信息

Unité Hormones et Cancer (U148) INSERM, Université de Montpellier 1, Département de Biologie Cellulaire, France.

出版信息

Nucleic Acids Res. 1993 Aug 11;21(16):3909-10. doi: 10.1093/nar/21.16.3909.

DOI:10.1093/nar/21.16.3909

PMID:8367316

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309934/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efcf/309934/00186538befa/nar00065-0299-a.jpg

相似文献

Excess PCR primers may dramatically affect SSCP efficiency.

Nucleic Acids Res. 1993 Aug 11;21(16):3909-10. doi: 10.1093/nar/21.16.3909.

One-step multiplex PCR strategy for identification of mutations by SSCP and DNA sequencing.

Biotechniques. 2000 Aug;29(2):234-6. doi: 10.2144/00292bm06.

Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.

Nucleic Acids Res. 1993 Jul 11;21(14):3333-4. doi: 10.1093/nar/21.14.3333.

Direct cycle sequencing of mutated alleles detected by PCR single-strand conformation polymorphism analysis.

Biotechniques. 1993 May;14(5):790-4.

Efficient PCR production of single-stranded DNA sequencing templates.

Methods Mol Biol. 1996;65:149-53. doi: 10.1385/0-89603-344-9:149.

Multiple fluorescence-based PCR-SSCP analysis.

Biotechniques. 1994 Feb;16(2):296-7, 300-5.

Direct DNA sequencing following SSCP analysis.

Anal Biochem. 1994 May 1;218(2):478-9. doi: 10.1006/abio.1994.1216.

Direct DNA sequencing of PCR products.

Curr Protoc Mol Biol. 2001 Nov;Chapter 15:Unit 15.2. doi: 10.1002/0471142727.mb1502s56.

Thermal cycle dideoxy DNA sequencing.

Methods Mol Biol. 1996;58:413-23. doi: 10.1385/0-89603-402-X:413.

Sequencing of double-stranded PCR products.

Methods Mol Biol. 1993;23:183-90. doi: 10.1385/0-89603-248-5:183.

引用本文的文献

Inheritance and molecular variations of PCR-SSCP fragments in pedunculate oak (Quercus robur L.).

Theor Appl Genet. 1996 Aug;93(3):348-54. doi: 10.1007/BF00223175.

Improvement of the sensitivity and resolution of PCR-SSCP analysis with optimized primer concentrations in PCR products.

J Genet. 2006 Dec;85(3):233-5. doi: 10.1007/BF02935339.

Combined SSCP/duplex analysis by capillary electrophoresis for more efficient mutation detection.

Nucleic Acids Res. 2001 Jul 15;29(14):E71. doi: 10.1093/nar/29.14.e71.

本文引用的文献

Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766-70. doi: 10.1073/pnas.86.8.2766.

Detecting single base substitutions as heteroduplex polymorphisms.

Genomics. 1992 Feb;12(2):301-6. doi: 10.1016/0888-7543(92)90377-5.

Protocols for an improved detection of point mutations by SSCP.

Nucleic Acids Res. 1991 Jul 25;19(14):4009. doi: 10.1093/nar/19.14.4009.

Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP.

Nucleic Acids Res. 1992 Feb 25;20(4):871-8. doi: 10.1093/nar/20.4.871.

Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s).

Nucleic Acids Res. 1992 Feb 11;20(3):573-9. doi: 10.1093/nar/20.3.573.

Mutation analysis of genetic diseases by asymmetric-PCR SSCP and ethidium bromide staining: application to neurofibromatosis and cystic fibrosis.

Mol Cell Probes. 1992 Oct;6(5):357-9. doi: 10.1016/0890-8508(92)90027-u.

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Excess PCR primers may dramatically affect SSCP efficiency.

作者信息

Cai Q Q, Touitou I

机构信息

Unité Hormones et Cancer (U148) INSERM, Université de Montpellier 1, Département de Biologie Cellulaire, France.

出版信息

Nucleic Acids Res. 1993 Aug 11;21(16):3909-10. doi: 10.1093/nar/21.16.3909.

DOI:10.1093/nar/21.16.3909

PMID:8367316

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309934/

Abstract

摘要

相似文献

Excess PCR primers may dramatically affect SSCP efficiency.

Nucleic Acids Res. 1993 Aug 11;21(16):3909-10. doi: 10.1093/nar/21.16.3909.

One-step multiplex PCR strategy for identification of mutations by SSCP and DNA sequencing.

Biotechniques. 2000 Aug;29(2):234-6. doi: 10.2144/00292bm06.

Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.

Nucleic Acids Res. 1993 Jul 11;21(14):3333-4. doi: 10.1093/nar/21.14.3333.

Direct cycle sequencing of mutated alleles detected by PCR single-strand conformation polymorphism analysis.

Biotechniques. 1993 May;14(5):790-4.

Efficient PCR production of single-stranded DNA sequencing templates.

Methods Mol Biol. 1996;65:149-53. doi: 10.1385/0-89603-344-9:149.

Multiple fluorescence-based PCR-SSCP analysis.

Biotechniques. 1994 Feb;16(2):296-7, 300-5.

Direct DNA sequencing following SSCP analysis.

Anal Biochem. 1994 May 1;218(2):478-9. doi: 10.1006/abio.1994.1216.

Direct DNA sequencing of PCR products.

Curr Protoc Mol Biol. 2001 Nov;Chapter 15:Unit 15.2. doi: 10.1002/0471142727.mb1502s56.

Thermal cycle dideoxy DNA sequencing.

Methods Mol Biol. 1996;58:413-23. doi: 10.1385/0-89603-402-X:413.

Sequencing of double-stranded PCR products.

Methods Mol Biol. 1993;23:183-90. doi: 10.1385/0-89603-248-5:183.

引用本文的文献

Inheritance and molecular variations of PCR-SSCP fragments in pedunculate oak (Quercus robur L.).

Theor Appl Genet. 1996 Aug;93(3):348-54. doi: 10.1007/BF00223175.

Improvement of the sensitivity and resolution of PCR-SSCP analysis with optimized primer concentrations in PCR products.

J Genet. 2006 Dec;85(3):233-5. doi: 10.1007/BF02935339.

Combined SSCP/duplex analysis by capillary electrophoresis for more efficient mutation detection.

Nucleic Acids Res. 2001 Jul 15;29(14):E71. doi: 10.1093/nar/29.14.e71.

本文引用的文献

Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766-70. doi: 10.1073/pnas.86.8.2766.

Detecting single base substitutions as heteroduplex polymorphisms.

Genomics. 1992 Feb;12(2):301-6. doi: 10.1016/0888-7543(92)90377-5.

Protocols for an improved detection of point mutations by SSCP.

Nucleic Acids Res. 1991 Jul 25;19(14):4009. doi: 10.1093/nar/19.14.4009.

Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP.

Nucleic Acids Res. 1992 Feb 25;20(4):871-8. doi: 10.1093/nar/20.4.871.

Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s).

Nucleic Acids Res. 1992 Feb 11;20(3):573-9. doi: 10.1093/nar/20.3.573.

Mutation analysis of genetic diseases by asymmetric-PCR SSCP and ethidium bromide staining: application to neurofibromatosis and cystic fibrosis.

Mol Cell Probes. 1992 Oct;6(5):357-9. doi: 10.1016/0890-8508(92)90027-u.