通过物理化学方法纯化马铃薯Y病毒普通株系（PVYo）及其可溶性抗原。
Purification of PVYo and its soluble antigen by physico chemical means.
作者信息
Polson A
机构信息
Department of Biochemistry, University of Stellenbosch, Tygerberg, Republic of South Africa.
出版信息
Prep Biochem. 1993 Feb-May;23(1-2):273-84. doi: 10.1080/10826069308544556.
Potato virus Yo was purified by centrifugation of infected and minced plant tissue in the virus extraction rotor. As the initial seeding material was heavily contaminated with tobacco mosaic virus (TMV) this virus was isolated and antibody was elicited in chickens. The chicken antibody (IgY) against TMV was used for removing this extraneous virus from the original PVYo seeding material prior to propagating PVYo in tobacco plants, CV Glutinosa.
通过在病毒提取转子中对感染并切碎的植物组织进行离心，纯化了马铃薯Y病毒（Potato virus Yo）。由于初始接种材料被烟草花叶病毒（TMV）严重污染，因此分离出该病毒并在鸡体内引发抗体。在烟草植物CV Glutinosa中繁殖PVYo之前，使用针对TMV的鸡抗体（IgY）从原始PVYo接种材料中去除这种外来病毒。
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