Wehner E P, Brendel M
Institute für Mikrobiologie der J.W. Goethe-Universität, Frankfurt am Main, Federal Republic of Germany.
Yeast. 1993 Jul;9(7):783-5. doi: 10.1002/yea.320090712.
Formaldehyde (FA), a chemical with low toxic potential, is used as sole selective agent for transformation in the yeast Saccharomyces cerevisiae. Neither stable auxotrophic markers in recipient cells nor defined synthetic media are needed when multicopy vector YFRp1, containing the yeast SFA gene, is employed for yeast transformation. The SFA gene gives stability to the vector and its yeast (and other) passenger genes when transformants are propagated in complex media supplemented with 3-5 mM-FA. Use of inexpensive FA and non-synthetic, undefined media will lower the cost of yeast transformant propagation considerably and thus make feasible large-volume industrial application of transformants containing YFRp1 derivatives.
甲醛（FA）是一种潜在毒性较低的化学物质，用作酿酒酵母转化的唯一选择剂。当使用含有酵母SFA基因的多拷贝载体YFRp1进行酵母转化时，受体细胞中既不需要稳定的营养缺陷型标记，也不需要特定的合成培养基。当转化体在添加了3 - 5 mM - FA的复合培养基中传代培养时，SFA基因赋予载体及其酵母（和其他）外源基因稳定性。使用廉价的FA和非合成、未定义的培养基将大大降低酵母转化体传代培养的成本，从而使含有YFRp1衍生物的转化体的大规模工业应用成为可能。
