Araki K, Kinoshita S, Kuwayama Y, Ohashi Y
Department of Ophthalmology, Osaka University Medical School, Japan.
Nippon Ganka Gakkai Zasshi. 1993 Aug;97(8):906-12.
We investigated the rate of corneal epithelial wound healing in rabbits which had undergone surgical trigeminal denervation. Seven New Zealand albino rabbits underwent unilateral neurotomy of postganglionic trigeminal nerves via intracranial route. At 14 days after operation, 10 mm diameter central corneal epithelial defects were created in both eyes by n-heptanol. The area of epithelial defect was then photographed at 12-hour intervals and the epithelial healing rate was calculated (1st experiment). At 14 days after epithelial wound closure, the corneal epithelium was again denuded in a similar fashion (2nd experiment). The epithelial healing rate of the denervated and control eyes respectively were 1.28 +/- 0.25 and 1.39 +/- 0.31 (mm2/hr) in the first experiment, and 1.05 +/- 0.20 and 1.44 +/- 0.22 (mm2/hr) in the second. There was a statistically significant difference in the epithelial healing rate between the two groups in experiment 2 (p < 0.05), These results indicate that corneal epithelial wound healing is impaired in the denervated eye.
我们研究了接受手术三叉神经去神经支配的兔子角膜上皮伤口愈合的速率。七只新西兰白化兔通过颅内途径接受了节后三叉神经的单侧神经切断术。术后14天，用正庚醇在双眼制造直径10毫米的中央角膜上皮缺损。然后每隔12小时拍摄上皮缺损区域，并计算上皮愈合速率（第一个实验）。在上皮伤口闭合后14天，以类似方式再次剥脱角膜上皮（第二个实验）。在第一个实验中，去神经支配眼和对照眼的上皮愈合速率分别为1.28±0.25和1.39±0.31（平方毫米/小时），在第二个实验中分别为1.05±0.20和1.44±0.22（平方毫米/小时）。在实验2中，两组之间的上皮愈合速率存在统计学上的显著差异（p<0.05）。这些结果表明，去神经支配眼的角膜上皮伤口愈合受到损害。
