Arcelloni C, Fermo I, Banfi G, Pontiroli A E, Paroni R
Scientific Institute H.S. Raffaele, Milan, Italy.
Anal Biochem. 1993 Jul;212(1):160-7. doi: 10.1006/abio.1993.1307.
The capillary zone electrophoresis (CZE) technique was evaluated for separation and quantification of human growth hormone (hGH), human insulin (hI), and proinsulin. Three different molecular forms of biosynthetic hGH (20K, 22K, 44K), methionyl-hGH, biosynthetic hI, and proinsulin, were studied. The hormones were separated with uncoated capillaries, and various analytical conditions were tested (different buffers, ionic strength, pH). The samples were introduced both at positive pressure and electrokinetically, and the voltage applied was varied in each case. Linearity, repeatibility, and limit of detection (0.8 microM for hGH and 1.3 microM for insulin) were determined. Different purification steps were tried in order to find a suitable preanalytical procedure for hGH and hI purification from plasma. Recovery rates from 65 to 80% were obtained.
对毛细管区带电泳（CZE）技术进行了评估，以分离和定量人生长激素（hGH）、人胰岛素（hI）和胰岛素原。研究了三种不同分子形式的生物合成hGH（20K、22K、44K）、甲硫氨酰-hGH、生物合成hI和胰岛素原。使用未涂层毛细管分离这些激素，并测试了各种分析条件（不同缓冲液、离子强度、pH值）。样品通过正压和电动方式进样，每种情况下施加的电压都有所不同。测定了线性、重复性和检测限（hGH为0.8 microM，胰岛素为1.3 microM）。尝试了不同的纯化步骤，以找到从血浆中纯化hGH和hI的合适分析前程序。回收率为65%至80%。
