DeGraves F J, Anderson K L
Department of Large Animal Surgery and Medicine, College of Veterinary Medicine, Auburn University, AL 36849.
Am J Vet Res. 1993 Jul;54(7):1128-32.
Ibuprofen treatment was compared with saline solution treatment in an endotoxin-induced experimental model of bovine mastitis. Acute mastitis was induced in healthy lactating Holstein cows (n = 12) by intramammary inoculation of 1 mg of Escherichia coli 026:B6 lipopolysaccharide in a single quarter per cow. Cows were assigned at random to ibuprofen (25 mg/kg of body weight, IV, n = 6) or 0.9% sodium chloride solution control (1.25 ml/kg, IV, n = 6) treatment groups. Ibuprofen or saline solution was administered once, 2 hours after endotoxin administration. The clinical course of endotoxin-induced mastitis and hematologic, clinical biochemical, and plasma mineral changes were monitored and compared between ibuprofen-treated and control cows. Clinical monitoring and blood sample collection were performed at 0, 2, 4, 6, 8, 12, 24, 48, 96, and 192 hours after endotoxin challenge. Rectal temperature and heart and respiratory rates were significantly (P < or = 0.05) increased in saline treated cows, compared with cows treated with ibuprofen. Blood eosinophil count and serum phosphorus, sodium, and total carbon dioxide concentrations were significantly (P < or = 0.05) decreased in saline-treated cows, compared with cows treated with ibuprofen. Ibuprofen treatment did not significantly change ruminations per minute, electrical conductivity of milk, quarter size, or quarter inflammation. The remaining hematologic, serum biochemical, plasma mineral, and coagulation values also were not changed significantly in response to ibuprofen treatment. Untoward effects attributed to ibuprofen administration were not observed. These results indicate that ibuprofen may provide empiric relief of clinical signs of coliform-induced mastitis.
在内毒素诱导的牛乳腺炎实验模型中，将布洛芬治疗与生理盐水治疗进行了比较。通过向健康的泌乳荷斯坦奶牛（n = 12）的每个乳房单季度内接种1毫克大肠杆菌026:B6脂多糖，诱导急性乳腺炎。奶牛被随机分配到布洛芬组（25毫克/千克体重，静脉注射，n = 6）或0.9%氯化钠溶液对照组（1.25毫升/千克，静脉注射，n = 6）治疗组。在内毒素给药2小时后，一次性给予布洛芬或生理盐水。对内毒素诱导的乳腺炎的临床病程以及血液学、临床生化和血浆矿物质变化进行监测，并在布洛芬治疗的奶牛和对照奶牛之间进行比较。在内毒素攻击后0、2、4、6、8、12、24、48、96和192小时进行临床监测和血样采集。与用布洛芬治疗的奶牛相比，生理盐水治疗的奶牛的直肠温度、心率和呼吸频率显著（P≤0.05）升高。与用布洛芬治疗的奶牛相比生理盐水治疗的奶牛的血液嗜酸性粒细胞计数以及血清磷、钠和总二氧化碳浓度显著（P≤0.05）降低。布洛芬治疗并未显著改变每分钟反刍次数、牛奶电导率、乳房大小或乳房炎症。其余的血液学、血清生化、血浆矿物质和凝血值也未因布洛芬治疗而发生显著变化。未观察到归因于布洛芬给药的不良影响。这些结果表明，布洛芬可能为大肠埃希菌引起的乳腺炎的临床症状提供经验性缓解。
