Urano F, Imoto M, Fukuda Y, Koyama K, Nakano I
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Arzneimittelforschung. 1993 Jul;43(7):804-7.
Using flow cytometric methods, the influence of recombinant human macrophage colony-stimulating factor (rhM-CSF) on DNA synthesis, the phagocytosis of microspheres and the uptake of lipopolysaccharide (LPS) in cultured rat Kupffer cells was investigated. Incubation with 10(3) U/ml of rhM-CSF did not increase the S-phase population of Kupffer cells, however, 10(5) U/ml of M-CSF significantly increased that population from 6.26 +/- 0.68% to 8.31 +/- 0.99%. Significantly increased rate of cells phagocytosing microspheres was provoked by rhM-CSF at concentration higher than 10(3) U/ml. Uptake indexes at concentrations of 10(3), 10(4), 10(5), and 10(6) U/ml of rhM-CSF were 114.2 +/- 7.7, 116.5 +/- 8.7, 117.5 +/- 9.1, and 120.0 +/- 11.5, respectively. Significantly increased rate of cells stained with fluorescent LPS was provoked by rhM-CSF at concentrations higher than 10(5) U/ml. Uptake indexes at concentrations of 10(5) and 10(6) U/ml were 115.2 +/- 6.4 and 118.0 +/- 4.3, respectively. These results indicate that M-CSF has a potency to stimulate DNA synthesis, phagocytic activity, and LPS uptake of mature Kupffer cells in vitro. This potency may be helpful to the enhancement or the recovery of the role of Kupffer cells, which is a part of the host defense mechanism, in diseased states.
采用流式细胞术方法，研究了重组人巨噬细胞集落刺激因子（rhM-CSF）对培养的大鼠库普弗细胞DNA合成、微球吞噬作用及脂多糖（LPS）摄取的影响。用10³ U/ml的rhM-CSF孵育并未增加库普弗细胞的S期细胞群，然而，10⁵ U/ml的M-CSF显著增加了该细胞群，从6.26±0.68%增至8.31±0.99%。rhM-CSF在浓度高于10³ U/ml时可显著提高细胞吞噬微球的速率。rhM-CSF浓度为10³、10⁴、10⁵和10⁶ U/ml时的摄取指数分别为114.2±7.7、116.5±8.7、117.5±9.1和120.0±11.5。rhM-CSF在浓度高于10⁵ U/ml时可显著提高细胞被荧光LPS染色的速率。浓度为10⁵和10⁶ U/ml时的摄取指数分别为115.2±6.4和118.0±4.3。这些结果表明，M-CSF具有刺激体外成熟库普弗细胞DNA合成、吞噬活性及LPS摄取的能力。这种能力可能有助于在疾病状态下增强或恢复作为宿主防御机制一部分的库普弗细胞的作用。
