Gillies K, Price D J
Department of Physiology, University Medical School, Edinburgh, UK.
Neuroreport. 1993 Jul;4(7):911-4. doi: 10.1097/00001756-199307000-00018.
We studied whether cortical cells migrate and subplate cells are lost in explants of murine cortex cultured for up to 14 days in defined serum-free medium. We gave bromodeoxyuridine (BrdU) to mice on embryonic days 12-13 (E12-13), to label subplate cells, or E17, to label cells destined for superficial cortical layers. We started culturing on E18-20. We left some mice to develop and provide in vivo data. At E18-20, most cells born on E17 lay below the cortical plate. During the following days in vitro, many of them moved to the cortical plate's superficial edge, although an abnormally high number remained in the ventricular zone. There was a selective loss of BrdU labelled cells from the subplate in culture; this occurred more rapidly than in vivo. Factors absent from our cultures may normally enhance migration and prolong the survival of subplate cells.
我们研究了在限定的无血清培养基中培养长达14天的小鼠皮质外植体中，皮质细胞是否迁移以及亚板层细胞是否丢失。我们在胚胎第12 - 13天（E12 - 13）给小鼠注射溴脱氧尿苷（BrdU）以标记亚板层细胞，或在E17注射以标记注定要进入皮质浅层的细胞。我们在E18 - 20开始培养。我们让一些小鼠发育以提供体内数据。在E18 - 20时，大多数在E17出生的细胞位于皮质板下方。在接下来的体外培养的日子里，它们中的许多细胞迁移到了皮质板的浅层边缘，尽管有异常高数量的细胞仍留在脑室区。培养物中亚板层中BrdU标记的细胞出现了选择性丢失；这种情况在体外比在体内发生得更快。我们培养物中缺乏的因子可能通常会促进迁移并延长亚板层细胞的存活时间。
