Escartin-Peña M, Bautista-Garfias C R
Centro Agropecuario, Posta Zootécnica, Universidad Autónoma de Aguascalientes, Morelos, México.
Med Vet Entomol. 1993 Jul;7(3):233-7. doi: 10.1111/j.1365-2915.1993.tb00682.x.
Sera from 41 horses and 159 donkeys, from twelve States of México, were tested to ascertain anti-Gasterophilus circulating antibodies by double immunodiffusion (DD), counterimmunoelectrophoresis (CIE), indirect haemagglutination (IH), thin layer immunoassay (TIA) and diffusion-in-gel ELISA (DIG-ELISA) methods using crude somatic antigen from third instar larvae of G. intestinalis (DeGeer). At necropsy, 33/41 horses and 24/159 donkeys were found to be parasitized by G. intestinalis and/or G. nasalis (L.). Gasterophilus intestinalis was the species most commonly found in the equines. Analysis of the sera from the infected animals by DD showed positive results of 21.2% in horses and of 8% in donkeys. Screening the sera with CIE gave sensitivities of 69.7% in horses and of 32% in donkeys. Examination of the sera by IH showed positive results of 87.9% and of 48% in horses and donkeys, respectively. Testing the sera with TIA gave sensitivities of 93.9% in horses and of 96% in donkeys. Analysis of horses' sera by DIG-ELISA showed a sensitivity of 93.9%.
对来自墨西哥12个州的41匹马和159头驴的血清进行了检测，采用双免疫扩散（DD）、对流免疫电泳（CIE）、间接血凝（IH）、薄层免疫测定（TIA）和凝胶扩散酶联免疫吸附测定（DIG-ELISA）方法，使用来自肠道胃蝇（DeGeer）三龄幼虫的粗制菌体抗原，以确定抗胃蝇属循环抗体。尸检时，发现33/41匹马和24/159头驴被肠道胃蝇和/或鼻胃蝇（L.）寄生。肠道胃蝇是在马属动物中最常见的种类。通过双免疫扩散对感染动物的血清进行分析，结果显示马的阳性率为21.2%，驴的阳性率为8%。用对流免疫电泳对血清进行筛查，马的敏感性为69.7%，驴的敏感性为32%。通过间接血凝检测血清，马和驴的阳性结果分别为87.9%和48%。用薄层免疫测定检测血清，马的敏感性为93.9%，驴的敏感性为96%。通过凝胶扩散酶联免疫吸附测定分析马的血清，敏感性为93.9%。
