James S, Richardson P J
Neurochem Int. 1993 Aug;23(2):115-22. doi: 10.1016/0197-0186(93)90088-m.
The subcellular distribution of the adenosine A2a receptor in rat striatum has been investigated using specific binding of the A2a-selective ligand [3H]-CGS 21680. After subcellular fractionation, the distribution of [3H]-CGS 21680 binding was similar to that of the cholinergic nerve terminal marker acetylcholinesterase rather than the more general membrane marker 5'-nucleotidase, with 42% of binding associated with the synaptosomal sub-fraction and 19% with a light membrane fraction. Binding of [3H]-CGS 21680 was also found to co-purify with the cholinergic nerve terminal marker choline acetyltransferase during immunoaffinity purification of striatal cholinergic nerve terminals. These results demonstrate that some adenosine A2a receptors are present on cholinergic nerve terminals in rat striatum.
利用 A2a 选择性配体[3H]-CGS 21680 的特异性结合，对大鼠纹状体中腺苷 A2a 受体的亚细胞分布进行了研究。亚细胞分级分离后，[3H]-CGS 21680 结合的分布与胆碱能神经末梢标志物乙酰胆碱酯酶的分布相似，而不是与更通用的膜标志物 5'-核苷酸酶的分布相似，42%的结合与突触体亚组分相关，19%与轻膜组分相关。在纹状体胆碱能神经末梢的免疫亲和纯化过程中，还发现[3H]-CGS 21680 的结合与胆碱能神经末梢标志物胆碱乙酰转移酶共同纯化。这些结果表明，大鼠纹状体的胆碱能神经末梢上存在一些腺苷 A2a 受体。
