利血平对大鼠脑内色氨酸羟化酶活性及mRNA的急性影响。

Acute effects of reserpine on tryptophan hydroxylase activity and mRNA in rat brain.

作者信息

Park D H, Wessel T C, Joh T H

机构信息

Laboratory of Molecular Neurobiology, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605.

出版信息

Brain Res. 1993 Aug 27;620(2):331-4. doi: 10.1016/0006-8993(93)90175-m.

DOI:10.1016/0006-8993(93)90175-m

PMID:8369965

Abstract

The present study examined changes in tryptophan hydroxylase (TPH) activity in the rat brainstem 1-4 days after i.p. reserpine administration. TPH enzyme activity was significantly decreased day 1 posttreatment and followed by a small but significant increase on days 2-4. Maximal TPH activity on day 4 was elevated only 42% above control level. This small increase in TPH activity was not preceded by any change of in situ hybridization signal of TPH message on day 1. In contrast, adrenal tyrosine hydroxylase (TH) activity in these same reserpinized animals was 2.5-fold higher than control on 4 day posttreatment. In addition, TH message was correspondingly increased day 1 posttreatment. Taken together, these results indicate a trend of small increase in TPH activity and no alteration in message after reserpine treatment unlike catecholaminergic systems.

摘要

本研究检测了腹腔注射利血平后1 - 4天大鼠脑干中色氨酸羟化酶（TPH）活性的变化。治疗后第1天TPH酶活性显著降低，随后在第2 - 4天有小幅但显著的升高。第4天TPH活性的最大值仅比对照水平高42%。TPH活性的这种小幅增加在第1天并没有伴随TPH信息原位杂交信号的任何变化。相比之下，在这些相同的利血平化动物中，肾上腺酪氨酸羟化酶（TH）活性在治疗后第4天比对照高2.5倍。此外，治疗后第1天TH信息相应增加。综上所述，这些结果表明，与儿茶酚胺能系统不同，利血平治疗后TPH活性有小幅增加的趋势，且信息没有改变。