Lightstone E, Marvel J, Mitchison A
Imperial Cancer Research Fund Tumour Immunology Unit, University College London, GB.
Eur J Immunol. 1993 Sep;23(9):2383-6. doi: 10.1002/eji.1830230952.
Mouse CD4 T cells have been partitioned into CD45RA and CD45RA- subpopulations by means of the monoclonal antibody 14.8. The CD45RA- subpopulation proliferated more actively and generated more interleukin-4 (IL-4) in response to stimulation with anti-CD3 antibody and phytohemagglutinin, and more IL-2 in response to anti-CD3. This subpopulation is therefore hyper-reactive to these polyclonal stimulators, but does not show the bias towards T helper type 2 activity that has been found in studies with other related CD45 isoforms. No evidence of suppression was obtained by comparing proliferation of CD45RA- cells in the presence and absence of CD45RA cells. Thus mouse CD4 T cells behave in these respects similarly to those of man, as is evident in a brief review of the quiescence-activation-quiescence cycle in the two species.
通过单克隆抗体14.8已将小鼠CD4 T细胞分为CD45RA和CD45RA-亚群。CD45RA-亚群在受到抗CD3抗体和植物血凝素刺激时增殖更为活跃，并产生更多的白细胞介素-4（IL-4），在受到抗CD3刺激时产生更多的IL-2。因此，该亚群对这些多克隆刺激物反应过度，但未表现出在其他相关CD45同种型研究中发现的对2型辅助性T细胞活性的偏向。通过比较存在和不存在CD45RA细胞时CD45RA-细胞的增殖情况，未获得抑制的证据。因此，小鼠CD4 T细胞在这些方面的行为与人的相似，这在对两个物种的静止-激活-静止周期的简要回顾中很明显。
