Burgering M J, Boelens R, Caffrey M, Breg J N, Kaptein R
Bijvoet Center for Biomolecular Research, Utrecht, The Netherlands.
FEBS Lett. 1993 Sep 6;330(1):105-9. doi: 10.1016/0014-5793(93)80929-o.
For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by double filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.
对于对称蛋白质二聚体的结构测定，区分亚基内和亚基间的核Overhauser效应（NOE）是必要的。本文提出了一种使用均匀的¹⁵N和¹³C同位素标记来选择性测量亚基间NOE的方法。这是通过对天然蛋白质与同位素标记蛋白质的混合物进行双滤波二维NOE实验来实现的。该方法已应用于Arc阻遏蛋白，并能够根据其亚基内或亚基间的性质对几乎所有质子-质子NOE进行表征。
