Colonna-Romano S, Patriarca E J, Amar M, Bernard P, Manco G, Lamberti A, Iaccarino M, Defez R
International Institute of Genetics and Biophysics, Naples, Italy.
FEBS Lett. 1993 Sep 6;330(1):95-8. doi: 10.1016/0014-5793(93)80927-m.
Permeabilization with cetyl trimethyl ammonium bromide was used to study the post-translational modification of the PII protein in Rhizobium leguminosarum. Upon incubation with radioactive UTP a single band was obtained after SDS-PAGE and autoradiography. RNase resistance and snake venom phosphodiesterase sensitivity showed that radioactivity was bound through a phosphodiester bond to a protein which was absorbed by an antiserum specific for the PII protein. Uridylylation of the PII protein was shown to be dependent on the modifications of the glutamine/alpha-ketoglutarate ratio.
采用十六烷基三甲基溴化铵通透法研究了豆科根瘤菌中PII蛋白的翻译后修饰。与放射性UTP孵育后，经SDS-PAGE和放射自显影得到一条单一的条带。核糖核酸酶抗性和蛇毒磷酸二酯酶敏感性表明，放射性通过磷酸二酯键与一种蛋白质结合，该蛋白质可被PII蛋白特异性抗血清吸收。结果表明，PII蛋白的尿苷酰化依赖于谷氨酰胺/α-酮戊二酸比率的修饰。
