戈登分枝杆菌中限制性内切酶MgoI的纯化与特性分析
Purification and characterization of restriction endonuclease MgoI from Mycobacterium gordonae.
作者信息
Shankar S, Tyagi A K
机构信息
Department of Biochemistry, University of Delhi South Campus, India.
出版信息
Gene. 1993 Sep 6;131(1):153-4. doi: 10.1016/0378-1119(93)90686-w.
PMID:8370536
Abstract
A restriction endonuclease, MgoI, an isoschizomer of Sau3AI, was purified from Mycobacterium gordonae TRC1318. As compared to Sau3AI, the yield of MgoI was seven-eightfold higher, the enzyme was four times more stable at 37 degrees C, and in addition, had optimal activity over a much broader range of salt concentrations.
摘要
一种限制性内切酶MgoI，即Sau3AI的同裂酶，是从戈登分枝杆菌TRC1318中纯化得到的。与Sau3AI相比，MgoI的产量高出七至八倍，该酶在37℃时的稳定性是Sau3AI的四倍，此外，在更广泛的盐浓度范围内具有最佳活性。
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