Targovnik H M, Frechtel G D, Varela V, Wajchenberg B L, Vassart G, Dumont J, Medeiros-Neto G A
Catedra de Genética y Biología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
J Endocrinol Invest. 1993 Jun;16(6):415-9. doi: 10.1007/BF03348869.
Thyroid tissue total RNAs from multinodular goiter (G2) and from hereditary goiter with defective Tg synthesis (JNA) were hybridized with a 5'albumin cDNA probe (F-47), a 3' albumin cDNA probe (B-44) and a thyroglobulin cDNA probe (phTgM3). JNA refers to tissue obtained from a patient with virtual absence of Tg in thyroid tissue and the presence of increased concentration of an albumin-like labeled protein in the thyroid. No hybridization signal was detected in both G2 and JNA with albumin probes at Northern Blot studies. Those results were confirmed by dot-blot analysis of total RNA where no hybridization signal was detected in G2 and JNA. To confirm that thyroid tissues do not express thyroalbumin total RNA from JNA and normal control thyroid tissue (C) were amplified by PCR using albumin and Tg primers. An expected fragment of 592 bp was observed in a human liver sample with the albumin primers. However JNA and C samples showed absence of an amplification product of the same size. We concluded that thyroid cells do not contain the albumin transcript. Albumin is probably taken up from circulation and iodinated by the thyroid follicular cell with subsequent release of iodoalbumin into the circulation.
来自多结节性甲状腺肿（G2）和伴有甲状腺球蛋白（Tg）合成缺陷的遗传性甲状腺肿（JNA）的甲状腺组织总RNA，与5'白蛋白cDNA探针（F - 47）、3'白蛋白cDNA探针（B - 44）和甲状腺球蛋白cDNA探针（phTgM3）进行杂交。JNA指的是从甲状腺组织中几乎不存在Tg且甲状腺中白蛋白样标记蛋白浓度升高的患者获得的组织。在Northern印迹研究中，G2和JNA与白蛋白探针均未检测到杂交信号。通过对总RNA的斑点印迹分析证实了这些结果，其中在G2和JNA中均未检测到杂交信号。为了确认甲状腺组织不表达甲状腺白蛋白，使用白蛋白和Tg引物通过PCR扩增JNA和正常对照甲状腺组织（C）的总RNA。用白蛋白引物在人肝样本中观察到预期的592 bp片段。然而，JNA和C样本均未显示相同大小的扩增产物。我们得出结论，甲状腺细胞不含有白蛋白转录本。白蛋白可能是从循环中摄取，并由甲状腺滤泡细胞碘化，随后碘白蛋白释放到循环中。
