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Restriction endonuclease Alw NI is blocked by overlapping Dcm methylation.

作者信息

Rieger A, Nassal M

机构信息

Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.

出版信息

Nucleic Acids Res. 1993 Aug 25;21(17):4148. doi: 10.1093/nar/21.17.4148.

DOI:10.1093/nar/21.17.4148
PMID:8371990
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310025/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a060/310025/ca953a77754b/nar00066-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a060/310025/ca953a77754b/nar00066-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a060/310025/ca953a77754b/nar00066-0223-a.jpg

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1
Restriction endonuclease Alw NI is blocked by overlapping Dcm methylation.限制性内切酶Alw NI会被重叠的Dcm甲基化所阻断。
Nucleic Acids Res. 1993 Aug 25;21(17):4148. doi: 10.1093/nar/21.17.4148.
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The inhibition of restriction endonuclease PvuII cleavage activity by methylation outside its recognition sequence.限制内切酶PvuII在其识别序列外甲基化导致的切割活性抑制。
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The Escherichia coli chromosome contains specific, unmethylated dam and dcm sites.大肠杆菌染色体含有特定的、未甲基化的dam和dcm位点。
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DNA cleavage by restriction endonuclease PflMI is inhibited in recognition sites modified by dcm methylation.限制内切酶PflMI对DNA的切割在被dcm甲基化修饰的识别位点中受到抑制。
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Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes.编码Sau96I限制酶和甲基化酶的基因的克隆及核苷酸序列
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Cleavage by the restriction endonuclease Asp718, an isoschizomer of KpnI, is sensitive to Escherichia coli Dcm methylation.限制性内切酶Asp718(KpnI的同裂酶)的切割对大肠杆菌Dcm甲基化敏感。
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Methylation of DNA in prokaryotes.原核生物中DNA的甲基化。
EXS. 1993;64:39-108. doi: 10.1007/978-3-0348-9118-9_4.
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Cleavage by ApaI is inhibited by overlapping dcm methylation.ApaI的切割会被重叠的dcm甲基化所抑制。
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The great GATC: DNA methylation in E. coli.伟大的GATC:大肠杆菌中的DNA甲基化
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A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation.一种基于微生物转化后DNA环化提高环状DNA构建效率的策略。
Microb Cell Fact. 2015 Feb 12;14:18. doi: 10.1186/s12934-015-0204-x.

本文引用的文献

1
The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function.乙肝病毒RNA前基因组上的包装信号形成一个茎环结构,这对其功能至关重要。
Nucleic Acids Res. 1993 Aug 25;21(17):3967-75. doi: 10.1093/nar/21.17.3967.
2
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
3
Total chemical synthesis of a gene for hepatitis B virus core protein and its functional characterization.
乙型肝炎病毒核心蛋白基因的全化学合成及其功能表征。
Gene. 1988 Jun 30;66(2):279-94. doi: 10.1016/0378-1119(88)90364-2.
4
Cleavage by EcoO109 and DraII is inhibited by overlapping dcm methylation.EcoO109和DraII的切割会受到重叠的dcm甲基化的抑制。
Nucleic Acids Res. 1988 Feb 25;16(4):1623. doi: 10.1093/nar/16.4.1623.
5
Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化:对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2045-71. doi: 10.1093/nar/19.suppl.2045.
6
Restriction enzymes: properties and use.限制性内切酶:特性与应用
Methods Enzymol. 1992;216:199-224. doi: 10.1016/0076-6879(92)16023-d.
7
Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.新型克隆载体的构建与特性研究。III. 携带独特Eco RI位点的质粒pBR322衍生物,用于筛选由Eco RI产生的重组DNA分子。
Gene. 1978 Oct;4(2):121-36. doi: 10.1016/0378-1119(78)90025-2.
8
Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.大肠杆菌K12中DNA腺嘌呤甲基化突变(dam-3)的多效性作用
Mutat Res. 1975 Apr;28(1):15-26. doi: 10.1016/0027-5107(75)90309-7.