Susić V, Markovic O
Department of Physiology, Medical School, University of Belgrade, Serbia.
Physiol Behav. 1993 Aug;54(2):331-8. doi: 10.1016/0031-9384(93)90119-z.
The possibility that REM sleep deprivation (REMD) induced increased susceptibility of rats to the convulsive effects of metaphit was investigated. Metaphit-induced audiogenic seizures were studied in three groups of animals: 1) caged controls; 2) large platform animals; and 3) small platform, REMD animals. After 48 h of confinement to their environments the rats from all three groups were injected with metaphit (10 mg kg-1, IP) and the procedures continued for the next 24 h. Immediately after removal from platforms and at 3-h intervals thereafter all rats were individually subjected to intense sound stimulation. Convulsive responses were recorded and analyzed with respect to incidence, intensity, and duration. The REMD rats were found to be more sensitive to the convulsive effects of metaphit compared to nondeprived rats. This was manifested in significantly shorter latencies to seizures, and significantly higher incidence, severity, and duration of seizures, especially of the most severe seizure component-tonic extensor convulsion. Inducing rats to convulse while they were being REM sleep deprived eliminated the REM sleep rebound observed in REMD rats that did not convulse. The occurrence of spontaneous EEG seizures during the undisturbed recovery period reduced REM sleep rebound. The results demonstrate a reciprocal relation between seizure behavior and REM sleep.
研究了快速眼动睡眠剥夺（REMD）是否会导致大鼠对美加明惊厥作用的易感性增加。在三组动物中研究了美加明诱发的听源性惊厥：1）笼养对照；2）大平台动物；3）小平台快速眼动睡眠剥夺动物。在限制于各自环境48小时后，给三组大鼠均腹腔注射美加明（10毫克/千克），并在接下来的24小时内持续该操作。从平台上取下后立即以及此后每隔3小时，对所有大鼠分别进行强烈声音刺激。记录惊厥反应，并就发生率、强度和持续时间进行分析。发现与未剥夺快速眼动睡眠的大鼠相比，快速眼动睡眠剥夺大鼠对美加明的惊厥作用更敏感。这表现为惊厥潜伏期显著缩短，惊厥的发生率、严重程度和持续时间显著更高，尤其是最严重的惊厥成分——强直性伸展性惊厥。在快速眼动睡眠剥夺期间诱导大鼠惊厥消除了未惊厥的快速眼动睡眠剥夺大鼠中观察到的快速眼动睡眠反弹。在未受干扰的恢复期出现的自发性脑电图惊厥减少了快速眼动睡眠反弹。结果表明惊厥行为与快速眼动睡眠之间存在相互关系。
