High-level production of an active single-chain Fv fragment in the culture supernatant of Escherichia coli.
作者信息
Sizmann D, Takkinen K, Laukkanen M L, Saloheimo M, Candussio A, Veijola-Bailey P, Teeri T T
机构信息
VTT Biotechnical Laboratory, Espoo, Finland.
出版信息
Year Immunol. 1993;7:119-30.
PMID:8372501
Abstract
摘要
相似文献
1
High-level production of an active single-chain Fv fragment in the culture supernatant of Escherichia coli.
Year Immunol. 1993;7:119-30.
2
[Design of secretion vectors for Escherichia coli].
Mol Biol (Mosk). 1996 Jan-Feb;30(1):111-20.
3
pSKAP/S: An expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins.pSKAP/S：用于生产单链Fv碱性磷酸酶融合蛋白的表达载体。
Protein Expr Purif. 1999 Jun;16(1):63-9. doi: 10.1006/prep.1999.1041.
4
Gene cloning, bacterial expression, in vitro refolding, and characterization of a single-chain Fv antibody against PreS1(21-47) fragment of HBsAg.抗乙肝表面抗原前S1（21-47）片段单链Fv抗体的基因克隆、细菌表达、体外重折叠及鉴定
Protein Expr Purif. 2005 Jun;41(2):341-8. doi: 10.1016/j.pep.2005.02.005.
5
[Expression, structure prediction and functional analysis of murine single-chain fragment variable antibody against human cervical cancer].[抗人宫颈癌小鼠单链可变片段抗体的表达、结构预测及功能分析]
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jan;26(1):16-21.
6
Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297.球形芽孢杆菌2297营养生长阶段产生的一种杀蚊幼虫毒素的克隆与特性分析
Curr Microbiol. 2004 Aug;49(2):84-8. doi: 10.1007/s00284-004-4274-y.
7
Generation and application of a fluorescein-specific single chain antibody.一种荧光素特异性单链抗体的产生与应用。
Biochimie. 2007 Nov;89(11):1304-11. doi: 10.1016/j.biochi.2007.06.008. Epub 2007 Jul 1.
8
[Construction and expression of single chain Fv antibody against human bladder carcinoma].[抗人膀胱癌单链Fv抗体的构建与表达]
Zhonghua Wai Ke Za Zhi. 2001 Oct;39(10):792-5.
9
Production of recombinant proteins: challenges and solutions.重组蛋白的生产：挑战与解决方案。
Methods Mol Biol. 2004;267:15-52. doi: 10.1385/1-59259-774-2:015.
10
Using secretion to solve a solubility problem: high-yield expression in Escherichia coli and purification of the bacterial glycoamidase PNGase F.利用分泌作用解决溶解性问题：在大肠杆菌中高效表达及纯化细菌糖基酰胺酶PNGase F
Protein Expr Purif. 2002 Feb;24(1):90-8. doi: 10.1006/prep.2001.1555.
Zotero 插件