Fahrenholz F, Jurzak M, Gerstberger R, Haase W
Max-Planck-Institute of Biophysics, Frankfurt, Germany.
Ann N Y Acad Sci. 1993 Jul 22;689:194-206. doi: 10.1111/j.1749-6632.1993.tb55548.x.
Employing an anti-vasopressin monoclonal antibody for immunization, anti-idiotypic monoclonal antibodies were obtained which induced plasminogen activator production in the renal epithelial cell line LLC-PK1. The anti-idiotypic antibodies were employed to visualize vasopressin receptors on LLC-PK1 and A7r5 smooth muscle cells by immunofluorescence. All results indicated specificity of the anti-idiotypes for both V1 and V2 vasopressin receptor subtypes. These antibodies were used for immunohistochemical localization of vasopressin receptors in rat and bovine kidney preparations. In accordance with earlier physiological and biochemical observations, vasopressin receptors were detected predominantly in collecting ducts in cortex and medulla. On the cellular level, a differential staining pattern was observed. On rat brain tissue sections, dense staining was observed within various circumventricular organs. The staining pattern corresponded to that obtained in autoradiographic studies with labeled AVP(4-9) fragment peptide and differed from the distribution of binding sites for labeled vasopressin or V1 antagonists.
利用抗血管加压素单克隆抗体进行免疫，获得了抗独特型单克隆抗体，这些抗体可诱导肾上皮细胞系LLC-PK1产生纤溶酶原激活物。利用抗独特型抗体通过免疫荧光观察LLC-PK1和A7r5平滑肌细胞上的血管加压素受体。所有结果表明抗独特型抗体对血管加压素V1和V2受体亚型均具有特异性。这些抗体用于大鼠和牛肾脏标本中血管加压素受体的免疫组织化学定位。与早期的生理学和生物化学观察结果一致，血管加压素受体主要在皮质和髓质的集合管中检测到。在细胞水平上，观察到了不同的染色模式。在大鼠脑组织切片上，在各种室周器官内观察到密集染色。这种染色模式与用标记的AVP(4-9)片段肽进行放射自显影研究得到的结果一致，且与标记的血管加压素或V1拮抗剂结合位点的分布不同。
