Mendz G L, Lim T N, Hazell S L
School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.
Arch Biochem Biophys. 1993 Sep;305(2):252-60. doi: 10.1006/abbi.1993.1419.
Eight fluorinated compounds were tested as putative probes to measure carboxylesterase activity employing 19F nuclear magnetic resonance spectroscopy. The method takes advantage of the sensitivity of fluorine resonances to the changes in the chemical bonding in the covalent structures where they are located. Determination of the kinetic parameters of ethyl fluoroacetate and diethyl fluoromalonate in hemolysates showed that these probes were well suited to study carboxylesterase activities in complex systems the potential of these probes for noninvasive applications was demonstrated in measurements of carboxylesterase kinetic parameters in intact erythrocytes. The presence of carboxylesterases was established in several strains of the bacterium Helicobacter pylori employing 19F nuclear magnetic resonance spectroscopy, and the kinetic parameters of these enzymes for ethyl fluoroacetate and diethyl fluoromalonate were measured.
使用19F核磁共振光谱法对8种氟化化合物作为测量羧酸酯酶活性的假定探针进行了测试。该方法利用了氟共振对其所在共价结构中化学键变化的敏感性。对溶血产物中氟乙酸乙酯和氟代丙二酸二乙酯的动力学参数测定表明，这些探针非常适合研究复杂系统中的羧酸酯酶活性，在完整红细胞中羧酸酯酶动力学参数的测量中证明了这些探针用于非侵入性应用的潜力。利用19F核磁共振光谱法在几种幽门螺杆菌菌株中确定了羧酸酯酶的存在，并测量了这些酶对氟乙酸乙酯和氟代丙二酸二乙酯的动力学参数。
