大鼠肝脏过氧化物酶体中F1-ATP酶γ亚基的免疫学检测

Immunological detection of F1-ATPase gamma-subunit in rat liver peroxisomes.

作者信息

Imanaka T, Shiina Y, Moriyama Y, Ohkuma S, Takano T

机构信息

Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.

出版信息

Biochem Biophys Res Commun. 1993 Sep 15;195(2):1027-34. doi: 10.1006/bbrc.1993.2147.

DOI:10.1006/bbrc.1993.2147

PMID:8373382

Abstract

The gamma-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as alpha, beta and delta were not detected in the peroxisomes. The polypeptide corresponding to the gamma-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase gamma-subunit. The amount of gamma-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of alpha, beta and delta-subunits were not increased. These results suggest that the gamma-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.

摘要

利用针对纯化线粒体F1 - ATP酶的抗血清，通过免疫印迹分析在高度纯化的大鼠肝脏过氧化物酶体中检测到了F1 - ATP酶的γ亚基。在过氧化物酶体中未检测到F1 - ATP酶的其他亚基，如α、β和δ亚基。过氧化物酶体中与γ亚基相对应的多肽也被针对F1 - ATP酶γ亚基的亲和纯化抗体所识别。在大鼠服用氯贝丁酯后，肝脏匀浆中γ亚基的量随着过氧化物酶体的增殖而平行增加，而α、β和δ亚基的量并未增加。这些结果表明，F1 - ATP酶的γ亚基是大鼠肝脏过氧化物酶体的一个组成部分。