Akiyama N, Sasaki H, Katoh O, Sato T, Hirai H, Yazaki Y, Sugimura T, Terada M
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1993 Sep 15;195(2):1041-9. doi: 10.1006/bbrc.1993.2149.
To study the involvement of cyclins in cell-cycle progression, changes of mRNA levels for three G1 cyclins (cyclin C, D1 and E) and cyclin A were studied in a leukemia cell line, HEL cells, before and after incubation with 12-O-tetradecanoylphorbol-13-acetate (TPA). Unexpectedly, the cyclin D1 mRNA level markedly increased in the HEL cells when the cells were growth-arrested by TPA, while the amounts of cyclin E and A mRNAs decreased to an almost undetectable level in HEL cells after incubation with TPA. The similar marked increment of the cyclin D1 mRNA level was observed in other leukemia cell lines, CMK and HL-60 cells, after incubation with TPA. The cyclin C mRNA was not detected in HEL cells before and after incubation with TPA.
为研究细胞周期蛋白在细胞周期进程中的作用，在白血病细胞系HEL细胞中，于12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）孵育前后，研究了三种G1期细胞周期蛋白（细胞周期蛋白C、D1和E）及细胞周期蛋白A的mRNA水平变化。出乎意料的是，当HEL细胞被TPA诱导生长停滞时，细胞周期蛋白D1的mRNA水平显著升高，而在与TPA孵育后，HEL细胞中细胞周期蛋白E和A的mRNA量降至几乎检测不到的水平。在用TPA孵育后，在其他白血病细胞系CMK和HL - 60细胞中也观察到细胞周期蛋白D1的mRNA水平有类似的显著升高。在与TPA孵育前后的HEL细胞中均未检测到细胞周期蛋白C的mRNA。
