Sawada T, Ho J J, Sagabe T, Yoon W H, Chung Y S, Sowa M, Kim Y S
Gastrointestinal Research Laboratory, Veterans Affairs Medical Center, San Francisco, CA 94121.
Biochem Biophys Res Commun. 1993 Sep 15;195(2):1096-103. doi: 10.1006/bbrc.1993.2157.
Partial removal of cell surface sialic acids, by enzymatic hydrolysis or by reducing sialylation, increased both adhesion to Matrigel and cellular aggregation in the pancreatic cancer cell line, SW1990. In contrast, a greater reduction in cell surface sialic acids decreased cellular adhesiveness below that of untreated cells. Cellular adhesion and homotypic cellular aggregation were enhanced by reducing O-linked glycosylation. Addition of purified SW1990 mucins reversed the effect of the inhibition of O-glycosylation. Both adhesion to extracellular matrix proteins and cellular aggregation were generally unaffected or decreased by inhibition of N-linked glycosylation. These results suggest that although certain sialic acids are required for the adhesion, sialic acids on oligosaccharides O-linked to surface glycoproteins inhibit SW1990 cellular aggregation and adhesion to the substratum.
通过酶促水解或减少唾液酸化对细胞表面唾液酸进行部分去除，可增加胰腺癌细胞系SW1990对基质胶的黏附以及细胞聚集。相反，细胞表面唾液酸的更大程度减少会使细胞黏附性降至未处理细胞以下。减少O-连接糖基化可增强细胞黏附和同型细胞聚集。添加纯化的SW1990黏蛋白可逆转O-糖基化抑制的作用。抑制N-连接糖基化通常对细胞与细胞外基质蛋白的黏附以及细胞聚集无影响或使其减少。这些结果表明，虽然某些唾液酸是黏附所必需的，但与表面糖蛋白O-连接的寡糖上的唾液酸会抑制SW1990细胞聚集和对基质的黏附。
