Bielinski D F, Pyun H Y, Linko-Stentz K, Macara I G, Fine R E
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Biochim Biophys Acta. 1993 Sep 19;1151(2):246-56. doi: 10.1016/0005-2736(93)90109-d.
We have employed high-resolution SDS polyacrylamide gels to demonstrate that there are two major low-molecular-weight GTP-binding proteins associated with axonal membranes including synaptic vesicles, rapid transported membranes and clathrin-coated vesicles. We demonstrate that one of the major proteins is Ral and that the other is Rab3A. Following the depolarization of synaptosomes resulting in increased neurotransmitter release, we see no significant dissociation of either Ral or Rab3a from synaptic vesicle derived membranes in contrast to results reported previously.
我们采用了高分辨率的SDS聚丙烯酰胺凝胶来证明，与轴突膜相关的有两种主要的低分子量GTP结合蛋白，包括突触小泡、快速运输膜和网格蛋白包被小泡。我们证明其中一种主要蛋白是Ral，另一种是Rab3A。在突触体去极化导致神经递质释放增加后，与之前报道的结果相反，我们没有看到Ral或Rab3a从突触小泡衍生膜上有明显解离。
