Lehmann A R, Kirk-Bell S, Arlett C F, Harcourt S A, de Weerd-Kastelein E A, Keijzer W, Hall-Smith P
Cancer Res. 1977 Mar;37(3):904-10.
Postreplication repair of DNA damage after ultraviolet light irradiation has been examined in a wide variety of human fibroblast strains. The donors were patients with xeroderma pigmentosum (XP) of different complementation groups or other hereditary disorders with indications of radiosensitivity, or with light sensitivity or multiple cancers. The defect in postreplication repair previously found in XP variants (excision-proficient XP's) has now been observed in a total of five XP variants and a less severe defect in postreplication repair has been found in excision-defective XP's in Complementation Groups A, B, C, and D. Complementation Group E and all other cell strains studied showed a response that was not significantly different from that of cells from normal donors. Excision repair was also measured in some of these cell strains and was found to be defective only in XP cells. Ultraviolet cell survival characteristics have been obtained for may of the cell strains. The most sensitive were cells from the excision-deficient XP's and from a sun-sensitive child (11961); the latter had no measurable defect in either excision or postreplication repair. The rest of the survival curves lay in a band limited by normal cell strains on the one hand and the slightly more sensitive excision-proficient XP variant XP30RO. Only in the case of the variants XP30RO and XP7TA were we able to demonstrate any influence of caffeine on cell survival.
已在多种人类成纤维细胞株中检测了紫外线照射后DNA损伤的复制后修复情况。供体为不同互补组的着色性干皮病（XP）患者或其他有放射敏感性、光敏感性或多发性癌症迹象的遗传性疾病患者。先前在XP变异体（切除功能正常的XP患者）中发现的复制后修复缺陷，现已在总共5个XP变异体中观察到，并且在A、B、C和D互补组中切除缺陷型XP患者中发现了不太严重的复制后修复缺陷。E互补组和所有其他研究的细胞株显示出与正常供体细胞的反应无显著差异。还在其中一些细胞株中测量了切除修复，发现仅在XP细胞中有缺陷。已获得许多细胞株的紫外线细胞存活特征。最敏感的是来自切除缺陷型XP患者和一名对阳光敏感儿童（11961）的细胞；后者在切除或复制后修复方面均无可测量的缺陷。其余的存活曲线位于一方面由正常细胞株、另一方面由稍敏感的切除功能正常的XP变异体XP30RO限定的范围内。仅在变异体XP30RO和XP7TA的情况下，我们能够证明咖啡因对细胞存活有任何影响。
