Aguado L I, Benelbaz G A, Gutierrez L S, Rodriguez E M
Cell Tissue Res. 1977 Jan 5;176(1):131-42. doi: 10.1007/BF00220349.
Pineal glands were grafted under the kidney capsule of mature male rats for periods of 20, 40, 60 and 100 days. Each grafted gland was then excised and divided into two halves. One half was processed for conventional electron microscopy and the other was fixed in aldehydes and then incubated in a zinc-iodide-osmium tetroxide mixture at pH 4.4 (A-ZIO-4.4). During the forty days following the operation pinealocytes showed the typical ultrastructural features associated with cells with a high protein and/or peptide secretory activity. On the other hand, during this period, the number of granular vesicles decreased progressively. From day 40 on, the grafted pinealocytes lacked granular vesicles. During the second half of the experimental period the ultrastructure of the pinealocytes indicated that their secretory activity was considerably decreased. During the acute phase of the experimental period numerous structures regarded as the tip of growing axons as well as typical nerve fibres appeared around blood vessels and within the paren chyma of the grafted gland. In the transplanted tissue obtained 60 and 100 days after the operation the growth cones were scarce, whereas typical nerve endings became numerous. These endings contained small clear vesicles which reacted positively when the tissue was treated with A-ZIO-4.4. The secretory activity of the grafted pineal gland and the nature of the nerve fibres which innervate the graft are discussed.
将松果体移植到成年雄性大鼠的肾被膜下，移植时间分别为20天、40天、60天和100天。然后将每个移植的松果体切除并分成两半。一半用于常规电子显微镜检查，另一半用醛类固定，然后在pH值为4.4的碘化锌-四氧化锇混合物（A-ZIO-4.4）中孵育。术后40天内，松果体细胞呈现出与具有高蛋白和/或肽分泌活性的细胞相关的典型超微结构特征。另一方面，在此期间，颗粒小泡的数量逐渐减少。从第40天开始，移植的松果体细胞缺乏颗粒小泡。在实验期的后半段，松果体细胞的超微结构表明其分泌活性显著降低。在实验期的急性期，在血管周围和移植腺体的实质内出现了许多被认为是生长轴突末端的结构以及典型的神经纤维。在术后60天和100天获得的移植组织中，生长锥稀少，而典型的神经末梢增多。这些末梢含有小的清亮小泡，当用A-ZIO-4.4处理组织时，这些小泡呈阳性反应。本文讨论了移植松果体的分泌活性以及支配移植体的神经纤维的性质。
