Nicholson W A, Ashton B A, Höhling H J, Quint P, Schreiber J, Ashton I K, Boyde A
Cell Tissue Res. 1977 Feb 15;177(3):331-45. doi: 10.1007/BF00220309.
The electron microprobe microanalyser has been used to measure the concentrations of Ca, P and S in the predentine of young rat incisors. The specimens were prepared as alcohol embedded ultrathin sections, unfixed vacuum embedded dry cut ultrathin sections and as thin cryostat sections. The results show the influence of preparation on the measured compositions and indicate that Ca is tightly bound to the matrix, whereas P can be easily washed out. Measurements along the dentine-predentine border demonstrated zones of Ca enrichment, the average size of which suggests that the zones could be the prestages of calcospherites. A mineralisation mechanism is discussed in which the high Ca concentration activates pyrophosphosphatase or ATPase before the onset of nucleation.
电子微探针微量分析仪已被用于测量幼鼠切牙前期牙本质中钙、磷和硫的浓度。标本制备成乙醇包埋超薄切片、未固定真空包埋干切超薄切片以及冰冻切片。结果显示了制备方法对所测成分的影响，并表明钙与基质紧密结合，而磷很容易被洗脱。沿牙本质-前期牙本质边界的测量显示出钙富集区，其平均大小表明这些区域可能是钙球晶的前期阶段。文中讨论了一种矿化机制，即在成核开始前，高钙浓度激活焦磷酸酶或ATP酶。
