Roberts M R, Hodge R, Ross J H, Sorensen A, Murphy D J, Draper J, Scott R
Department of Botany, University of Leicester, UK.
Plant J. 1993 May;3(5):629-36.
The expression and sequences of two related antherspecific cDNAs (I3 and C98) of Brassica napus (oilseed rape) were examined. These cDNAs were found to exhibit significant predicted amino acid sequence similarity with a group of seed-specific proteins, the oleosins, which are involved in oil body membrane structure. Pollen of B. napus also contains oil bodies, and the synthesis and accumulation of these organelles correlates with expression of the I3 and C98 transcripts. The protein content of purified pollen oil bodies was therefore examined in order to determine the presence or absence of possible oleosins. One major protein species of 14 kDa was identified and subjected to N-terminal amino acid sequence determination. The sequence of this pollen oil body associated protein is homologous to the predicted sequence of one of the anther-specific cDNs, I3, implying that this cDNA represents an anther-specific oleosin gene expressed in developing pollen. The expression of other genes active during seed oil body formation was also examined, and these were found to be specific to the sporophyte, inferring the existence of specific components of the pathway of lipid synthesis and storage in the male gametophyte. These possibilities and the relationship between different oleosin genes are discussed.
对甘蓝型油菜（油菜籽）两个相关的花药特异性cDNA（I3和C98）的表达和序列进行了研究。发现这些cDNA与一组种子特异性蛋白油质蛋白具有显著的预测氨基酸序列相似性，油质蛋白参与油体膜结构。甘蓝型油菜的花粉也含有油体，这些细胞器的合成和积累与I3和C98转录本的表达相关。因此，对纯化的花粉油体的蛋白质含量进行了检测，以确定是否存在可能的油质蛋白。鉴定出一种14 kDa的主要蛋白质，并对其进行了N端氨基酸序列测定。这种花粉油体相关蛋白的序列与其中一个花药特异性cDNA I3的预测序列同源，这意味着该cDNA代表一个在发育中的花粉中表达的花药特异性油质蛋白基因。还检测了在种子油体形成过程中活跃的其他基因的表达，发现这些基因对孢子体具有特异性，这表明雄配子体中存在脂质合成和储存途径的特定成分。讨论了这些可能性以及不同油质蛋白基因之间的关系。
