Parvy P, Bardet J, Rabier D, Gasquet M, Kamoun P
Laboratoire de Biochimie Médicale B, Hôpital Necker-Enfants Malades, Paris, France.
Clin Chem. 1993 Sep;39(9):1831-6.
The functioning of an external quality-control scheme for amino acids set up in 1978 is described. Two measurements were made each month by participating laboratories on a control plasma sample provided by the quality-control center; freeze-dried samples were used from 1978 to 1989 and liquid samples since 1990. In addition, two "blind" samples were sent to the laboratories each year. Every 3 months, the overall results and those of the individual laboratories were analyzed statistically. The validity of the liquid sample control is demonstrated. The progressive improvement of results is commendable. In 1990, the coefficients of variation for all participants ranged from 8.4% for alanine to 23.5% for methionine. The standards used for calibration could contribute to the broad range of results, especially those for histidine and ornithine. The use of blind samples made it possible to detect problems of calibration, of linearity of measurement, of contamination, and of identification of unusual amino acids.
本文描述了1978年建立的氨基酸外部质量控制计划的运行情况。参与实验室每月对质量控制中心提供的一份对照血浆样本进行两次检测；1978年至1989年使用冻干样本，1990年起使用液体样本。此外，每年向各实验室发送两份“盲样”。每3个月对总体结果和各实验室的结果进行统计分析。证明了液体样本对照的有效性。结果的逐步改善值得称赞。1990年，所有参与者的变异系数范围从丙氨酸的8.4%到蛋氨酸的23.5%。用于校准的标准可能导致结果范围较宽，尤其是组氨酸和鸟氨酸的结果。使用盲样使得检测校准问题、测量线性问题、污染问题以及异常氨基酸的识别成为可能。
