Arnott D, Shabanowitz J, Hunt D F
Department of Chemistry, University of Virginia, Charlottesville 22903.
Clin Chem. 1993 Sep;39(9):2005-10.
The study of proteins and peptides by mass spectrometry has been advanced by the development of matrix-assisted laser desorption/ionization and electrospray ionization. Proteins with masses > 100 kDa may be measured accurately at picomole sensitivities. Sequence analysis of peptides in mixtures at subpicomole quantities is possible by tandem mass spectrometry. Practical applications of the new technology to biological research are illustrated.
基质辅助激光解吸/电离和电喷雾电离技术的发展推动了通过质谱法对蛋白质和肽的研究。质量大于100 kDa的蛋白质可以在皮摩尔灵敏度下进行精确测量。通过串联质谱法可以对亚皮摩尔量混合物中的肽进行序列分析。文中举例说明了这项新技术在生物学研究中的实际应用。
