通过放射性气相色谱法测定皮质醇生成率。

Cortisol production rate determined by radio gas-chromatography.

作者信息

Derks H J, Muskiet F A, Wolthers B G, Thijssen J H, Drayer N M

出版信息

Clin Chem. 1977 Mar;23(3):518-21.

Abstract

We describe a ratio gas-chromatographic method for determination of cortisol production rate by measuring the isotope dilution of urinary cortisol metabolites. The method was calibrated by analyzing [3H]tetrahydrocortisol and [3H]tetrahydrocortisone of known specific activities. Results are reasonably well reproducible, the coefficients of variation ranging from 8-15% of allotetrahydrocortisol/tetrahydrocortisol and from 9-19% for tetrahydrocortisone. Correlation coefficients were 0.966 and 0.998 for tetrahydrocortisone and allotetrahydrocortisol/tetrahydrocortisol, respectively, when the method was compared with a method involving thin-layer chromatography and colorimetry. Only one chromatographic step is needed for both purification and quantitation, thus time and effort are saved.

摘要

我们描述了一种通过测量尿皮质醇代谢物的同位素稀释来测定皮质醇产生率的比率气相色谱法。该方法通过分析已知比活度的[3H]四氢皮质醇和[3H]四氢可的松进行校准。结果具有较好的重现性，全四氢皮质醇/四氢皮质醇的变异系数为8 - 15%，四氢可的松的变异系数为9 - 19%。当该方法与涉及薄层色谱和比色法的方法进行比较时，四氢可的松和全四氢皮质醇/四氢皮质醇的相关系数分别为0.966和0.998。纯化和定量只需一个色谱步骤，从而节省了时间和精力。