Liu T Z, Khayam-Bashi H
Clin Chem. 1977 Mar;23(3):581-3.
We examined the effect of sodium azide on the quantitation of serum uric acid by the direct acid ferric reduction procedure. Ferric phenanthroline was used as redox indicator. Sodium azide, in a concentration commonly used as preservative (2 g/liter, 27.2 mmol/liter), increased the absorption at 505 nm and increased apparent uric acid values in specimens, as shown by calculations based on an azide-free standard. Spectral studies indicated that this interference was a result of the color produced by sodium azide in the reaction mixture. The mechanism for azide interference was the interaction of sodium azide and ferric ions to form ferric azide, which also absorbs extensively at 505 nm.
我们通过直接酸性铁还原法研究了叠氮化钠对血清尿酸定量的影响。以邻菲罗啉铁作为氧化还原指示剂。叠氮化钠采用常用的防腐剂浓度（2克/升，27.2毫摩尔/升），会增加505纳米处的吸光度，并使标本中的表观尿酸值升高，基于无叠氮化物标准品的计算结果表明了这一点。光谱研究表明，这种干扰是反应混合物中叠氮化钠产生颜色的结果。叠氮化物干扰的机制是叠氮化钠与铁离子相互作用形成叠氮化铁，叠氮化铁在505纳米处也有广泛吸收。
