Khayam-Bashi H, Sims C
Am J Clin Pathol. 1978 Apr;69(4):405-9. doi: 10.1093/ajcp/69.4.405.
Azide salts frequently are added as a preservative to biologic fluids and reagents, or are introduced into serum through accidental exposure, intoxication, or pharmaceuticals. Sodium azide can interfere with the quantitation of biochemical constituents in serum. Serum pools containing 0.2-9.0 mg/dl (3.42-153.9 mumol/l) total bilirubin, 0.2-5.0 mg/dl (3.42-94.1 mumol/l) direct bilirubin, and 175-313 mg/dl (4.55-8.14 mmol/l) cholesterol were analyzed using the SMA 12/60. Sodium azide was added in concentrations of 0.1-1.0% (13.6-136 mmol/l). Sodium azide in concentrations of 0.1% (13.6 mmol/l) or more reduced total and direct bilirubin values 60-100%. At concentrations above 0.5% (68 mmol/l), no bilirubin, or only a very small quantity, was measured. Sodium azide at concentrations above 0.05% (6.8 mmol/l) exerted a significant decreasing effect on serum cholesterol values. At 0.1% (13.6 mmol/l) or more, reductions in cholesterol values ranging from 30 to 85% were observed. These studies showed that sodium azide (0.1%, 13.6 mmol/l, or more) in the serum can result in falsely low bilirubin or cholesterol values.
叠氮盐经常作为防腐剂添加到生物流体和试剂中，或者通过意外接触、中毒或药物进入血清。叠氮化钠会干扰血清中生化成分的定量分析。使用SMA 12/60分析仪对总胆红素含量为0.2 - 9.0mg/dl（3.42 - 153.9μmol/l）、直接胆红素含量为0.2 - 5.0mg/dl（3.42 - 94.1μmol/l）以及胆固醇含量为175 - 313mg/dl（4.55 - 8.14mmol/l）的血清样本进行分析。添加叠氮化钠的浓度为0.1% - 1.0%（13.6 - 136mmol/l）。浓度为0.1%（13.6mmol/l）及以上的叠氮化钠会使总胆红素和直接胆红素值降低60% - 100%。浓度高于0.5%（68mmol/l）时，未检测到胆红素或仅检测到极少量胆红素。浓度高于0.05%（6.8mmol/l）的叠氮化钠对血清胆固醇值有显著降低作用。浓度为0.1%（13.6mmol/l）及以上时，观察到胆固醇值降低30% - 85%。这些研究表明，血清中的叠氮化钠（0.1%，13.6mmol/l及以上）会导致胆红素或胆固醇值假性降低。
