通过异硫氰酸荧光素标记的硫酸鱼精蛋白对大鼠肥大细胞进行荧光显微镜观察。
Fluorescence microscopical visualization of rat mast cells by means of fluoresceine isothiocyanate labeled protamine sulfate.
作者信息
Schnitzler S, Renner H, Steinhauser G, Fechner K
出版信息
Acta Biol Med Ger. 1978;37(3):509-12.
PMID:83758
Abstract
Using fluoresceine isothiocyanate labeled protamine sulfate it is possible to stain rat mast cell fractions of isolated periotoneal cells in a selective way. The cells are not degranulated, though they have lost their histamine completely. The method shows the dissociation of the mediator release and the extrusion of the granules.
摘要
使用异硫氰酸荧光素标记的硫酸鱼精蛋白，可以选择性地对分离的腹膜细胞中的大鼠肥大细胞组分进行染色。尽管细胞已完全失去组胺，但并未脱颗粒。该方法显示了介质释放与颗粒排出的分离。
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引用本文的文献
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Histamine release from rat mast cells induced by protamine sulfate and polyethylene imine.硫酸鱼精蛋白和聚乙烯亚胺诱导大鼠肥大细胞释放组胺。
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