化学异种肿瘤的细胞介导免疫——VI. 逆转录病毒env反义寡核苷酸处理细胞的效果。

Cell-mediated immunity to chemically xenogenized tumors--VI. The effect of cell treatment with retroviral env antisense oligonucleotides.

作者信息

Grohmann U, Binaglia L, Puccetti P, Fioretti M C

机构信息

Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.

出版信息

Int J Immunopharmacol. 1993 Jul;15(5):567-72. doi: 10.1016/0192-0561(93)90073-8.

DOI:10.1016/0192-0561(93)90073-8

PMID:8375938

Abstract

The occurrence of aberrant, retrovirus-related proteins is a common finding in immunogenic clones of the triazene-xenogenized L5178Y lymphoma line (L5178Y/DTIC). In clone D-cells, newly expressed 80 kDa antigens related to xenotropic murine leukemia virus env gene products induce specific humoral and cell-mediated responses and possess biologic activity in vivo. To further clarify the relationship between immunogenic properties of clone D and retroviral gene expression, tumor cells were treated in vitro with antisense oligonucleotides complementary to xenotropic and/or polytropic env sequences of murine leukemia virus. The cells were then assayed for expression of antigens recognized by humoral and cell-mediated responses with specificity for clone D. The results demonstrated that inhibition of env mRNA translation adversely affected the expression of immunogenic determinants in the xenogenized tumor cells.

摘要

在三氮烯异种源化的L5178Y淋巴瘤细胞系（L5178Y/DTIC）的免疫原性克隆中，异常的、逆转录病毒相关蛋白的出现是一个常见的发现。在克隆D细胞中，新表达的与嗜异性小鼠白血病病毒env基因产物相关的80 kDa抗原可诱导特异性体液免疫和细胞介导的免疫反应，并在体内具有生物学活性。为了进一步阐明克隆D的免疫原性特性与逆转录病毒基因表达之间的关系，用与小鼠白血病病毒嗜异性和/或多嗜性env序列互补的反义寡核苷酸在体外处理肿瘤细胞。然后检测这些细胞对克隆D具有特异性的体液免疫和细胞介导免疫反应所识别的抗原的表达。结果表明，env mRNA翻译的抑制对异种源化肿瘤细胞中免疫原性决定簇的表达产生了不利影响。