小鼠淋巴瘤L5178Y的内源性逆转录病毒gp70基因：药物介导免疫原性诱导后限制性片段多态性分析
Endogenous retroviral gp70 genes of the murine lymphoma L5178Y: analysis of restriction fragment polymorphism upon induction of drug-mediated immunogenicity.
作者信息
Grohmann U, Belladonna M L, Allegrucci M, Silla S, Schiaffella E, Fioretti M C, Puccetti P
机构信息
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
出版信息
Viral Immunol. 1994;7(4):155-67. doi: 10.1089/vim.1994.7.155.
Highly immunogenic ("xenogenized") tumor variants appear after treatment of murine lymphoma L5178Y with the triazene derivative DTIC, this phenomenon being associated with the appearance of structurally abnormal gp70 env proteins in the cell variants. In the present study, we have isolated and sequenced several PCR-amplified gp70 cDNA genes from L5178Y cells. One of the resulting clones was used as a probe in Southern and Northern analysis of parental and xenogenized cells. The results indicated that xenogenization of tumor cells is associated with changes in retroviral env sequences detectable at the genomic level.
用三氮烯衍生物达卡巴嗪（DTIC）处理小鼠淋巴瘤L5178Y后，会出现高免疫原性（“异种源化”）的肿瘤变体，这种现象与细胞变体中结构异常的gp70 env蛋白的出现有关。在本研究中，我们从L5178Y细胞中分离并测序了几个经聚合酶链反应（PCR）扩增的gp70 cDNA基因。所得克隆之一用作亲本细胞和异种源化细胞的Southern印迹分析和Northern印迹分析的探针。结果表明，肿瘤细胞的异种源化与在基因组水平可检测到的逆转录病毒env序列变化有关。
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