Wada H, Avelange-Macherel M H, Murata N
National Institute for Basic Biology, Okazaki, Japan.
J Bacteriol. 1993 Sep;175(18):6056-8. doi: 10.1128/jb.175.18.6056-6058.1993.
The desA gene of the cyanobacterium Synechocystis sp. strain PCC6803 was expressed in Escherichia coli, which does not contain any fatty acid desaturase. The product of the desA gene catalyzed the desaturation of fatty acids at the delta 12 position. This result demonstrates that desA is the structural gene for a delta 12 desaturase.
蓝藻聚球藻属PCC6803菌株的desA基因在不含任何脂肪酸去饱和酶的大肠杆菌中表达。desA基因的产物催化脂肪酸在Δ12位的去饱和反应。这一结果表明desA是一种Δ12去饱和酶的结构基因。