Schramm T M, McKinnon G E, Eadie M J
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Herston, Australia.
J Chromatogr. 1993 Jun 23;616(1):39-44. doi: 10.1016/0378-4347(93)80469-k.
A rapid and specific gas chromatographic method has been developed for the determination of plasma R(-)- and S(+)-vigabatrin concentrations. The method involves a double derivatisation step, chromatography on a megabore Chirasil-Val capillary column and thermionic specific detection. Concentrations in the range 1.0-200 micrograms/ml can be measured for R(-)-vigabatrin, and 0.5-100 micrograms/ml for S(+)-vigabatrin, using 250 microliters of plasma. The assay is suitable for pharmacokinetic studies and routine therapeutic drug monitoring in humans.
