Tanikawa M, Uzu M, Ohsawa Y, Fukushima M
Assay Technology Laboratory, Development and Technology Division, Chugai Phamaceutical Co. Ltd., Tokyo, Japan.
J Chromatogr. 1993 Jul 23;617(1):163-7. doi: 10.1016/0378-4347(93)80437-9.
A sensitive method for the determination of nicorandil, an anti-anginal drug, has been developed. The method involves solid-phase extraction of the drug and an internal standard using a Bond-Elut PH extraction column, liquid-liquid extraction with methyl acetate and dichloromethane, and reversed-phase high-performance liquid chromatography on a mu Bondapak C18 column with an ultraviolet detector. The limit of determination in plasma was 3 ng/ml and the intra-day coefficient of variation (n = 7) was less than 10%.
已开发出一种用于测定抗心绞痛药物尼可地尔的灵敏方法。该方法包括使用Bond-Elut PH萃取柱对药物和内标进行固相萃取，用乙酸甲酯和二氯甲烷进行液-液萃取，以及在配有紫外检测器的μ Bondapak C18柱上进行反相高效液相色谱分析。血浆中的测定限为3 ng/ml，日内变异系数（n = 7）小于10%。
