Tagawa K, Hayashi K, Mizobe M, Noda K
Pharmaceutics Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
J Chromatogr. 1993 Jul 23;617(1):95-103. doi: 10.1016/0378-4347(93)80426-5.
A method for the simultaneous determination of imidapril and its active metabolite in human plasma and urine has been developed using high-performance liquid chromatography with a fluorescent labelling reagent, 9-anthryldiazomethane. Imidapril and its active metabolite were extracted from human plasma and urine using a solid-phase extraction cartridge (Bond Elut C18). Two compounds in the eluate were derivatized with 9-anthryldiazomethane and purified with a solid-phase extraction cartridge (Bond Elut SI). The derivatives were analysed using high-performance liquid chromatography with fluorometry. The detection limits of imidapril and its active metabolite were 0.2 ng/ml in plasma and 10 ng/ml in urine. This method could be applied to the pharmacokinetic study of imidapril.
已开发出一种使用高效液相色谱法和荧光标记试剂9-蒽重氮甲烷同时测定人血浆和尿液中咪达普利及其活性代谢物的方法。咪达普利及其活性代谢物采用固相萃取柱（Bond Elut C18）从人血浆和尿液中萃取。洗脱液中的两种化合物用9-蒽重氮甲烷衍生化，并用固相萃取柱（Bond Elut SI）纯化。衍生物采用带荧光检测的高效液相色谱法进行分析。咪达普利及其活性代谢物在血浆中的检测限为0.2 ng/ml，在尿液中的检测限为10 ng/ml。该方法可应用于咪达普利的药代动力学研究。
